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Quantitative determination of amino acid profile in DBS by LC-MS/MS technique

  • Test objective: To determine the concentration of amino acids in the test material. This allows for an assessment of the body's amino acid supply in order to develop a diet that ensures adequate levels of amino acid intake over time:
    • elimination diet
    • vegan or vegeterian diet
    • sports training
    • amino acid supplementation (BCAA, Beta-alanine, methionine, tyrosine)
    • increased demand (pregnancy, growth period, cancer, convalescence)
  • Method of testing: High-performance liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS)
  • Analysed substances: Alanine (Ala), Arginine (Arg), Asparagine (Asn), Aspartic acid (Asp), Beta-alanine (bAla), Citrulline (Cit), Glutamic acid (Glu), Glutamine (Gln), Glycine (Gly), Histidine (His), Homoarginine (hArg), Isoleucine (Ile), Leucine (Leu), Lysine (Lys), Methionine (Met), Ornithine (Orn,) Phenylalanine (Phe), Proline (Pro), Sarcosine (Sar), Serine (Ser), Threonine (Thr), Tryptophan (Trp), Tyrosine (Tyr), Valine (Val), Taurine (Tau), Gamma-aminobutyric acid (GABA).
  • Type of test material: Dried blood spot, serum, plasma
  • Test category: DBS, Metabolomics