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Quantitative determination of vitamin D metabolites in DBS by LC-MS/MS technique

  • Test objective: To assess the body's vitamin D supply, including specifically:
    • differential diagnosis of the causes of rickets and osteomalacia, low-energy fractures unrelated to high-force trauma, i.e., calcium-phosphate disorders
    • monitoring of therapy/supplementation with vitamin D preparations
    • diagnosis of hypovitaminosis, hypervitaminosis, and vitamin D poisoning
    • indications for the determination of 25(OH)D are also intestinal malabsorption, nephrotic syndrome, liver disease, decreased muscle and bone mass, treatment with corticosteroids, antiepileptic drugs, and immunosuppressants.
  • Method of testing: High-performance liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS)
  • Analysed substances:
    • 25(OH)D3 (calcifediol, calcidiol, or 25-hydroxy vitamin D3)
    • 25(OH)D2 (25-hydroxy vitamin D2)
    • 24,25(OH)2D3 (24,25-dihydroxy vitamin D3)
    • Epi-25(OH)D3 an empirical form with reduced biological activity – it is measured, but due to lack of clinical interpretation is not presented on the result
  • Type of test material: Dried blood spot, serum, plasma.
  • Test category: DBS, Metabolomic