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Quantitative determination of the fatty acid profile in DBS by GC-FID

  • Test objective: Analysis of the fatty acid profile is an important element in the prevention and treatment of cardiovascular diseases, neurological disorders and diet-related diseases. The results of the study enable individual adjustment of the diet and/or supplementation, and thanks to the use of indicators (Omega-3 Index, Trans Fat Index, Omega-6: Omega-3 ratio, AA: EPA ratio) they show mutual proportions of fatty acids.
  • Method of testing: Gas Chromatography (GC) with Flame Ionization Detection (FID)
  • Analysed substances:
    • Omega-3 fatty acids: alfa-linolenic acid (ALA, 18:3n3), eicosapentaenoic acid (EPA, 20:5n3), docosapentaenoic acid-n3 (22:5n3), docosahexaenoic acid (DHA, 22:6n3)
    • Omega-6 fatty acids: linoleic acid (18:2n6), gamma-linolenic acid (18:3n6), eicosadienoic acid (20:2n6), dihomo-gamma-linolenic acid (20:3n6), arachidonic acid (AA, 20:4n6 ), docosatetraenoic acid (22:4n6), docosapentaenoic acid-n6 (22:5n6)
    • Cis-monounsaturated fatty acids: palmitoleic acid (16:1n7), oleic acid (18:1n9), eicosenoic acid (20:1n9), nervonic acid (24:1n9)
    • Saturated fatty acids: myristic acid (14:0), palmitic acid (16:0), stearic acid (18:0), arachidic acid (20:0), bohenic acid (22:0), lignoceric acid (24:0 0)
    • Trans fatty acids: trans-palmitooleic acid (16:1n7t), trans-oleic acid (18:1t), trans-linoleic acid (18:2n6t)
    • Indicators: Omega-3 Index, Trans Fat Index, Omega-6 : Omega-3 Ratio and AA : EPA
  • Type of test material: Dried blood spot
  • Test category: DBS